General Cell Based Assays
Eurofins Discoverx PathHunter Mouse LTB4R β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Mouse LTB4R β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure mLTB4R1 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Amsbio LLC Heparanase Assay Kit with Positive Control Enzyme
Heparanase Assay Kit with Enzyme as Positive Control
Sartorius iQue Membrane Integrity Kit
Three dye options gives you more ways to measure cell death.No wash protocol just add and read for fast easily automated workflow.Multiplex with other IntelliCyt kits like Cell Cycle for richer content.PrincipleThe Cell Membrane Integrity dyes are fluorescent molecules that are cell impermeant. Healthy cells with intact cell membranes will exclude the dyes. Unhealthy cells with compromised membranes will allow entry of the dye into the intracellular space. Upon entry to the cell the dye localizes to the nucleus and binds to DNA by intercalation.
Eurofins Discoverx PathHunter eXpress PTGFR U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0936E3CP5L)
The PathHunter PTGFR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. cloprostenol) based activation of PTGFR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PTGFR and EA-tagged β-Arrestin-2. Activation of PTGFR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress FPRL1 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0044E2CP2M)
The cAMP Hunter FPRL1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of FPRL1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type FPRL1, a naturally Gi/Go coupled GPCR. Upon activation of FPRL1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam LDL Uptake Assay Kit Cell-Based
LDL Uptake Assay Kit Cell-Based
The product is subject to the following: Abcam Restricted Use Statement
Montana Molecular Green Cilia-targeted cADDis cAMP Assay - 10mL
Measure real-time cAMP signaling kinetics in the primary cilia with this live cell fluorescent assay. Add 5HT6-tagged cADDis to your cells and measure robust signals the next day on a fluorescence imaging system. Decreasing green fluorescence indicates ciliary cAMP increase. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cilia-targeted cAMP sensor BacMam and 500mM sodium butyrate in H2O.
Eurofins Discoverx PathHunter eXpress mRXFP4 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0929E2MCP2M)
The PathHunter mRXFP4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. insulin-like 5, relaxin-3) based activation of mRXFP4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mRXFP4 and EA-tagged β-Arrestin-2. Activation of mRXFP4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery RPLEX HUM CK Beta 81/CCL23 ASY
The Human CK-beta-8-1/CCL23 DuoSet ELISA is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CK-beta-8-1/CCL23 in cell culture supernatants serum plasma and other biological fluids This immunoassay contains recombinant human CK-beta-8-1/CCL23 and antibodies raised against the recombinant factor allowing for the detection of natural and recombinant human CK-beta-8-1/CCL23 The ELISA has a sensitivity of 7 8 pg/mL and a detection range of 15 6-1000 pg/mL This product can be used to measure CK-beta-8-1/CCL23 levels for the study of chemokine biology and inflammation
Meso Scale Discovery R-PLEX Human IL-23R ASY
The Meso Scale Discovery R-PLEX Human IL-23R Assay is a multiplex immunoassay for measuring IL-23R in human serum plasma and cell culture supernatants It uses electrochemiluminescence detection on patterned arrays for high sensitivity and a wide dynamic range The kit includes a plate with capture antibodies detection antibodies labeled with an electrochemiluminescent compound and calibrator and control samples for standard curve generation and assay validation It quantifies IL-23R with high precision and reproducibility suitable for 96 tests on MSD platforms
Lonza Walkersville PPiLight Inorganic Pyrophosphate Assay, 500 Test
Inorganic pyrophosphate (also referred to as diphosphate, pyrophosphoric acid or PPi) is a small diphosphate molecule that is required as a substrate or is the product formed from a number of different enzymatic reactions. Enzymes that utilize PPi as a substrate may include phosphotransferases (EC 2.7) and pyrophosphatases (EC 3.6.1.1). Enzymes that generate PPi are more numerous and may include cyclases (EC 4.6.1), hydrolyses (EC 3), ligases (EC 6) and DNA polymerases such as DNA Polymerase theta (θ) encoded by POLQ. Benefits:Fast - Measure enzyme activity via pyrophosphate consumption in 1 hourSimple - The 2 step, luminescent assay doesn’t require radioactive substrates, beads, detection antibodies or modified substratesWide Detection Range - Sensitive to 0.02 µM with a linear range to 10 µMVersatile - Scalable to 96-, 384-, 1586-well formatsApplications:Measure activity of:PhosphotransferasesPyrophosphotasesCyclasesLigasesHydrolasesDNA Polymerases
Eurofins Discoverx InCELL Hunter eXpress PIM1 Kinase Binding Assay, 2pl Kit. (96-0052E15CP0M)
The InCELL Hunter eXpress PIM1 Kinase Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with kinases. This kit assesses compound-engagement-based stabilization of PIM1 via detection of PIM1 levels. This product uses EFC technology. The cell line included overexpresses PIM1 target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter™ Human DRD5 Gs Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human DRD5 Gs Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure DRD5 (GPCR) activity via increased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.